The Importance of Identifying and Correctly Measuring Total (Adrenal) Estrogen

Posted on May 23rd, 2016

In the late 1940’s, one of the prominent human laboratories made the decision, that the body of the patient, will only produce three types of estrogen, which were estradiol, estrone and estriole.

These estrogens were produced mainly by the ovaries, and a small amount of these estrogens, were also produced by the brain, skin and fatty tissue.

They also believed that if any estrogen was produced by the inner layer adrenal cortex, it would be these same, three types of estrogen, but only in negligible amounts.

My clinical studies of 90,000 blood tests in animals and 2,000 blood tests in humans, indicates that this is not true, and that a large amount of adrenal estrogen is produced by the inner layer adrenal cortex, referred to as the Zona Reticularis, when the cortisol production from the middle layer adrenal cortex, referred to as the Zona Fasciculata is deficient, or non-functional.

Unfortunately, cortisol measurements are empirical and if the cortisol level is within the normal limits pf what that particular laboratory indicates as normal, that level of cortisol will still not indicate, whether the cortisol is functional or non-functional.

The only way to determine if a cortisol that is being produced, is functional or non-functional, is to measure adrenal estrogen, which can be tested for, by most humans laboratories and one veterinary laboratory, as total estrogen.

All the animal blood tests were performed on males and on females, without ovaries. When these animals were producing deficient or non-functional amounts of cortisol, it caused them to produce large amounts of adrenal estrogen.

In human males and in human females, that have had their ovaries removed,

They can still produce small amounts of estradiol, estrone and estriole from their skin, brain and fatty tissue.

There was no ovarian tissue present in these human patients, and because their measured cortisol was either deficient or non-functional, their adrenal estrogens

became elevated.

The elevation in adrenal estrogen is very significant, because elevated levels of estrogen, have been incriminated in causing allergies, autoimmunity and cancer, and yet, adrenal estrogen has not be included with an overproduction of estrogen, no matter, in what from it may appear.

PI personally believe that deficient or non-functional cortisol, which leads to elevated amounts of adrenal estrogen is the CAUSE of most chronic diseases like severe allergies, autoimmunity and cancer.

For an example of this in humans, on this website, please go to, The Results of an Integrative Conference for MD Oncologists, Regarding Dr. Plechner’s Findings for Many different, Human Diseases.

Adrenal estrogen unfortunately, is not being considered or even measured.

My clinical studies indicate that adrenal estrogen is different from estradiol, estrone and estriole.

To be able to identify an ACCURATE level of adrenal estrogen, National Veterinary Diagnostic Services, has provided the following directions, which can be used for handling, both human and animal blood samples.

. The blood sample ideally, should be taken 3 to 4 hours after eating, and before taking any medication.

. Any repeat blood tests, should be done at the same time of the day, to help avoid any unnecessary variations in the results..

. If alcohol is used to sterilize the area for the blood draw, the alcohol must be dry, otherwise if the alcohol is still wet, it will cause unnecessary hemolysis, of the serum.

. The blood sample should go into a serum separator, plastic, blood tube.

. Once the blood sample goes into the serum separator tube, it needs to be placed on its side in a refrigerated state, for 15 minutes. This procedure is done, in order to enhance the surface area of the blood sample and help create better clotting.

. If the centrifuged sample contains hemolysis, please send it to the laboratory in a refrigerated state, and not in a frozen state.

. If the centrifuged sample indicates no hemolysis, the sample can be sent to the laboratory, in a frozen state.

. Please place the centrifuged sample in a plastic bag that is separate from the paper, laboratory form.

. If the sample is to be mailed overnight, from a healthcare, professional’s office, the sample must remain refrigerated, in order for the test results to remain accurate. The sample must be mailed overnight on Monday through Thursday, otherwise if the sample is mailed on a Friday, it may not be delivered to the laboratory until Monday, and the sample will be ruined.

If serum sample is to the United States for processing, from a foreign country, dry ice may suffice.

Please realize, if a serum sample remains unrefrigerated, for a certain period of time, all the hormone and antibody values will become erroneously elevated.

If the sample has not been taken at an accredited laboratory, the centrifuged sample cannot remain in the centrifuge, unrefrigerated too long, otherwise the sample will yield falsely, high values, when processed.

If serum samples are collected in batches and remain at room temperature for a while, before they are processed, the serum values will be falsely elevated.

. If the collected serum samples are refrigerated without their rubber stopper in place, evaporation may occur, which will once again, will yield falsely, elevated values when the serum is processed.

By not paying attention to the details that are necessary in order to measure ACCURATE values for hormones and for antibodies, all the values may be elevated, and a true deficiency will not be identified, in your patient.

There are already established values for total (adrenal) estrogen, even if total estrogen, is rarely measured.

The following are those established values for total estrogen in humans and for canines and felines;

1) Human males = 80 to 115 pg/ml

2) Postmenopausal women = 40 pg/ml or less.

3) Menstruating women = at this time, in these clinical studies, to determine the difference between the amounts of ovarian and adrenal hormone, the following procedure may help to determine this;

A) Measure total estrogen at the end of the first week of the menstrual cycle, when the ovaries are producing the least amount of estrogen.

B) Remeasure the total estrogen at the time of the menstrual cycle, when the ovaries ate producing the most estrogen.

This may occur from the 23rd to the 25th day of the menstrual cycle, but this must be decided by each individual, themselves.

Hopefully the difference in the two types of estrogen, may indicate the amount of estrogen that is being produced by the inner layer adrenal cortex.

Hopefully more realistic means will be identified and applied in the future.

4) Canine and Feline males = 20 to 25 pg/ml.

5) Canine and Feline females = 30 to 35 pg/ml

Hopefully this article will indicate to you, that large amounts of adrenal estrogen will be produced, when the patient’s cortisol is deficient or non-functional.

Please remember, if a cortisol level is elevated, without first checking adrenal estrogen, there is no way to determine if the cortisol that is being produced, is functional or non-functional.

At this time in veterinary medicine, a patient is often subjected to having a test done for Cushing’s syndrome, which measures active cortisol.

The Cushing’s syndrome test should never be done without, first testing for levels of adrenal estrogen produced in the patient, because if the adrenal estrogen levels are above normal, it means the patient is producing a non-functional cortisol, and definitely does not have Cushing’s syndrome.

It is an unnecessary expense for the pet owner, and an unnecessary test for the pet.

Humans, canines and felines that have autoimmune diseases, will NOT have Cushing’s syndrome, which produces functional cortisol, which is used to treat many autoimmune diseases.

If you or your pet have allergies, autoimmunity or cancer, please asked you healthcare professional, to at least, measure your cortisol and total estrogen, just as a beginning.

For further information on the complete test procedures, please go to the internet and type in either the HUMAN PROTOCOL or the ANIMAL PROTOCOL, with my name.

Hopefully this will help create some original thinking, particularly for you and your pet, if either one of you have allergies, autoimmune diseases or cancer.


Dr. AL Plechner